Research

 

Chemical biology of nucleic acids. Our research aims at a comprehensive molecular understanding of important biological processes of nucleic acids, such as ribosomal translation and gene regulation by riboswitches. The Micura group has a strong focus on the chemical synthesis of ribonucleic acids (RNA) and on nucleic acid structure and function evaluation. In particular, the various aspects of chemically modified RNA - including naturally occuring as well as artificial modifications - are in the center of our research interests.

 

       Our methodological repertoire includes:

  • chemical synthesis of modified nucleosides and their corresponding phosphoramidites and triphosphates
  • RNA solid-phase synthesis
  • enzymatic RNA synthesis
  • chemical and enzymatic ligation techniques
  • HPLC and gel electrophoresis purification and analysis
  • UV-, fluorescence and CD-spectroscopy
  • LC-ESI-mass spectrometry
  • NMR spectroscopy
  • X-ray analysis of RNA (in collaborations)
  • molecular biology of RNA (in collaborations)